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TCS SP5 is currently the most confocal scanning microscope with the highest resolution. It makes full use of the most advanced technology concept and integrates it into a system. High precision Z axis stepping motor SuperZ - can quickly and parallel movement within the 1.5mm range, record 3D samples accurately and accurately, and make optical slicing; confocal special objective lens - flat field complex, high transmittance, and ultraviolet correction lens to meet the needs of full spectral imaging and co location analysis; from UV to IR. Stored in a system - three laser ports, which can integrate ultraviolet (351364, 405nm), visible, and infrared lasers into a system, covering a variety of stimulated luminescence from 350~1080nm, large scanning field of vision, high resolution images - using the X2Y patent scanning technology to provide a uniform large field of view of the 21.2mm; the image resolution is up to 819 2 x 8192; prism separation, spectral detection - prism separation, the transmittance is far higher than the grating. The spectral detector can choose the detection wavelength arbitrarily within the range of 400~800nm, which is suitable for any known, unknown fluorescent dyes and spontaneous fluorescence detection and spectral scanning, and the detection of the sensitivity is high. Multi channel imaging can be equipped with 5 water-cooled photomultiplier (PMT R9624), and 5 spectral fluorescent channel images are collected at the same time; 1 transmission light detection channels can be collected at the same time with the fluorescence channel image. The software - LAS AF software interface is intuitionistic and simple in operation. The basic drawing module has multi-dimensional data acquisition, multi channel simultaneous scanning, channel sequence scanning, region of interest scanning, emission spectrum recording, series color separation, image processing, quantitative analysis and so on. 3D imaging, CO location analysis, deconvolution, puzzle, dynamic physiology, FRAP, FRET and other software modules can meet the needs of different applications. The possibility of upgrading can be upgraded from a high resolution scanning system or a high-speed scanning system to a dual system; a two-photon system; a FCS (fluorescence correlation) system; a spectral FLIM (Ying Guangshou) The ROI spectrometer spectrometer system.
major function
Leica TCS SP5 MP
The infrared laser can be directly coupled to cover the 690~1040nm range.
The penetration depth is up to 500 m, which is very suitable for thick sample observation.
The non focal plane is not excited, so there is no bleaching phenomenon, and the damage to the sample is small.
With Z axis depth compensation function, the signal attenuation caused by the thickness of the sample can be corrected.
EOM adjusts the output power of laser, and 0~100% is continuously adjustable. It supports scanning of interested region, and is convenient for photoactivation, cage locking and bleaching.
To minimize the effect of fluorescent coloring;
The spectral detector or direct detector (NDD) can be used to receive fluorescence signals. The transmission (TLD) and the reflection direct detector (RLD) are installed near the concentrator and the object lens respectively. The light path is short and the maximum degree of light signal is collected, and the maximum can be matched with 4 direct detectors.
The spectral detector and direct detector are R9624 PMT, high sensitivity, high dynamic range and low dark current.
Confocal special objective lens;
It can collect images of up to 8 fluorescent channels and equipped with 1 transmission field detection channels.
High precision Z axis stepper motor, SuperZ, has a range of 250 m and a resolution of 10nm. It is fully qualified for fast 3D imaging.
Real confocal images with resolution up to 8192x8192 and high resolution can be obtained.
Matching temperature control and CO2 control system can realize long time observation of living cells.
Using LAS AF software platform, 3D, deconvolution, CO location, color separation and other software modules can be allocated.
Leica DM6000 CFS nerve and developmental biology research tool
The integration of SP5 MP Tandem system and CCD is the best platform for the observation of neurobiological samples; the conversion of confocal drawing and CCD drawing requires only one button.
High numerical aperture 20 x water mirror - HCX PL APO 20 x /1.0W, working distance 2mm, focusing range 13mm, suitable for thick sample observation; 39 degree operation angle is convenient for electrophysiological operation.
The 20 * water glass can be interchanged with the 6 bit objective turntable and can be equipped with various objective lenses.
The CCD interface multiplier can carry out 3 shift conversion of 0.35 x, 1 x and 4 x to facilitate the detailed observation of the large field of view and the regions of interest.
It can be equipped with transmission and reflection NDD detector. The optical path is short, the photon collection rate is high, and the acquisition sensitivity is high.
XY fixer table ensures the stability and reliability of the results.
Dodt observation method, without prism, can get similar DIC observation effect.
The intelligent touch panel STP6000 can control all functions of the microscope. It can freely define the function keys and operate the interface intuitively.
The trigger signal can be introduced or elicited to ensure the synchronization of morphological observation and electrophysiological measurement, and the optical and electrophysiological data can be obtained at the same time on the software, and the on-line analysis can be carried out.
It can be equipped with LAS AF software modules
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